Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Han, L.; Marcus, E.; DSilva, S.; Phizicky, E.
    S. cerevisiae Trm140 has two recognition modes for 3-methylcytidine modification of the anticodon loop of tRNA substrates (2017), RNA, 23, 406-419 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.268 Ses1 seryl-tRNA synthetase Ses1 greatly stimulates m3C modification of tRNASer(CGA) and tRNASer(UGA) in vitro Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.268 gene ABP140, recombinant expression in Saccharomyces cerevisiae, coexpression with yeast enzyme Ses1 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.268 additional information construction of a deletion trm140DELTA strain Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.268 cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.268 additional information Saccharomyces cerevisiae Trm140 interacts with Ses1, a cytoplasmic serine-tRNA ligase (EC 6.1.1.11). Ses1 stimulates m3C formation of tRNASer species ?
-
-
2.1.1.268 additional information Saccharomyces cerevisiae ATCC 204508 Trm140 interacts with Ses1, a cytoplasmic serine-tRNA ligase (EC 6.1.1.11). Ses1 stimulates m3C formation of tRNASer species ?
-
-
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer Saccharomyces cerevisiae ATCC 204508
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr Saccharomyces cerevisiae
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr Saccharomyces cerevisiae ATCC 204508
-
 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.268 Saccharomyces cerevisiae Q08641
-
-
2.1.1.268 Saccharomyces cerevisiae ATCC 204508 Q08641
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.268 additional information Trm140 interacts with Ses1, a cytoplasmic serine-tRNA ligase (EC 6.1.1.11). Ses1 stimulates m3C formation of tRNASer species Saccharomyces cerevisiae ?
-
-
2.1.1.268 additional information substrate specificity of enzyme Trm140 in vitro and in vivo, overview. An XGU anticodon and t6A37 are necessary and sufficient for m3C modification of tRNAThr species. tRNAThr(CGU) scaffold used for analysis of tRNAThr variants is fully functional and efficiently modified to m3C. For the tRNAThr(CGU) 28,50 variants, substitution of G35 or U36 with each of the other three nucleotides almost completely abolishes the copurification of tRNA, whereas substitution of C34 with other nucleotides leads to high levels of variant copurification. Trm140 binds tRNAs with a G35-U36 anticodon. G35 and U36 of tRNAThr(CGU) are required for m3C formation. Trm140 specifically binds tRNAThr substrates, activities with tRNAThr variants, overview Saccharomyces cerevisiae ?
-
-
2.1.1.268 additional information Trm140 interacts with Ses1, a cytoplasmic serine-tRNA ligase (EC 6.1.1.11). Ses1 stimulates m3C formation of tRNASer species Saccharomyces cerevisiae ATCC 204508 ?
-
-
2.1.1.268 additional information substrate specificity of enzyme Trm140 in vitro and in vivo, overview. An XGU anticodon and t6A37 are necessary and sufficient for m3C modification of tRNAThr species. tRNAThr(CGU) scaffold used for analysis of tRNAThr variants is fully functional and efficiently modified to m3C. For the tRNAThr(CGU) 28,50 variants, substitution of G35 or U36 with each of the other three nucleotides almost completely abolishes the copurification of tRNA, whereas substitution of C34 with other nucleotides leads to high levels of variant copurification. Trm140 binds tRNAs with a G35-U36 anticodon. G35 and U36 of tRNAThr(CGU) are required for m3C formation. Trm140 specifically binds tRNAThr substrates, activities with tRNAThr variants, overview Saccharomyces cerevisiae ATCC 204508 ?
-
-
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer best tRNASer substrate is tRNASer(UGA), lower activity with tRNASer(GCU) and tRNASer(CGA) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer
-
 Saccharomyces cerevisiae ATCC 204508 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNASer best tRNASer substrate is tRNASer(UGA), lower activity with tRNASer(GCU) and tRNASer(CGA) Saccharomyces cerevisiae ATCC 204508 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNASer
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr
-
 Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr
-
 Saccharomyces cerevisiae ATCC 204508 S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr
-
 ?
2.1.1.268 S-adenosyl-L-methionine + cytosine32 in tRNAThr G35 and U36 of tRNAThr(CGU) are required for m3C formation. Trm140 specifically binds tRNAThr substrates, overview. Best tRNAThr substrate is tRNATyr(GUA), lower activity occurs with tRNAThr(CGU), tRNAThr(IGU), and tRNAThr(UGU) Saccharomyces cerevisiae S-adenosyl-L-homocysteine + N3-methylcytosine32 in tRNAThr(CGU)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.268 Trm140
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.268 S-adenosyl-L-methionine
-
 Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.1.1.268 evolution in the yeast Saccharomyces cerevisiae, formation of m3C32 requires Trm140 for six tRNA substrates, including three tRNAThr species and three tRNASer species, whereas in Schizosaccharomyces pombe, two Trm140 homologues are used, one for tRNAThr and one for tRNASer. The occurrence of a single Trm140 homologue is conserved broadly among Ascomycota, whereas multiple Trm140-related homologues are found in metazoans and other fungi Saccharomyces cerevisiae
2.1.1.268 additional information enzyme Trm140 has two recognition modes for 3-methylcytidine modification of the anticodon loop of tRNA substrates. Trm140 recognizes G35-U36-t6A37 of the anticodon loop of tRNAThr substrates, and this sequence is an identity element because it can be used to direct m3C modification of tRNAPhe. Trm140 recognition of tRNASer substrates is different, since their anticodons do not share G35-U36 and do not have any nucleotides in common. Rather, specificity of Trm140 for tRNASer is achieved by seryl-tRNA synthetase and the distinctive tRNASer V-loop, as well as by t6A37 and i6A37 Saccharomyces cerevisiae
2.1.1.268 physiological function the 3-methylcytidine (m3C) modification is ubiquitous in eukaryotic tRNA, widely found at C32 in the anticodon loop of tRNAThr, tRNASer, and some tRNAArg species, as well as in the variable loop (V-loop) of certain tRNASer species. In the yeast Saccharomyces cerevisiae, formation of m3C32 requires Trm140 for six tRNA substrates, including three tRNAThr species and three tRNASer species. Trm140 binding is a significant driving force for tRNA modification and suggest separate contributions from each recognition element for the modification Saccharomyces cerevisiae